Israeli Paper on Efficient Pre-processing of PE Reads is Now Published

Israeli Paper on Efficient Pre-processing of PE Reads is Now Published

About a year back, we covered an NGS-related poster from an Israeli group -

An Efficient Preprocessing Module for Joining Paired end Reads before Assembly

@genetics_blog reported about a Bioinformatics paper from the authors that could be related to the poster. Unfortunately, the Bioinformatics paper is locked for a year or so. So, we will update here in April 2014, whether the paper is indeed the same as the poster. In the meanwhile, enjoy the abstract, which is open access.

ELOPER: elongation of paired-end reads as a pre-processing tool for improved de novo genome assembly

Motivation: Paired-end sequencing resulting in gapped short reads is commonly used for de novo genome assembly. Assembly methods use paired-end sequences in a two-step process, first treating each read-end independently, only later invoking the pairing to join the contiguous assemblies (contigs) into gapped scaffolds. Here, we present ELOPER, a pre-processing tool for pair-end sequences that produces a better read library for assembly programs.

Results: ELOPER proceeds by simultaneously considering both ends of paired reads generating elongated reads. We show that ELOPER theoretically doubles read-lengths while halving the number of reads. We provide evidence that pre- processing read libraries using ELOPER leads to considerably improved assemblies as predicted from the LanderWaterman model.


Speaking of ways to join PE reads, BGI also published on the same topic few months back. You will find the link in this blog post.

COPE (for Joining PE Reads) and Arapan-S (for Small Genomes)

Written by M. //