Richard Smith-Una, whose work was covered in our blog, releases a new quality assessment program (transrate) that we surely like to check out.
Transcriptome assembly is hard. The algorithms are complex, the data are messy, and it’s often not clear how to determine whether an assembly is suitable for answering a biological question.
Transrate helps by examining your assembly in detail and providing insight about its quality and usefulness in various situations. This can allow you to choose between assemblers and parameters, and helps you decide when to stop trying to improve the assembly.
Transrate analyses an assembly in three key ways:
by inspecting the contigs themselves
by mapping reads to the contigs and inspecting the alignments
by aligning the contigs against proteins from a related species and inspecting the alignments
You can read about these analyses in detail on the transcriptome assembly metrics page.
If you’ve got Ruby v2.0.0 or later, simply install Transrate with the command:
$ gem install transrate
Transrate depends on some external tools that can’t be installed by the transrate installer, including BLAST+ and Bowtie2. You can install these dependencies yourself, or you can let Transrate do it for you by running the command:
$ transrate –install-deps
If you don’t have at least v2 Ruby installed, you should install the latest version using the Ruby Version Manager: RVM.io, then install transrate as above.
The command-line interface
Running transrate –help will show you the command-line interface:
Transrate v0.2.0 by Richard Smith-Unna
and Chris Boursnell.
Analyse a de-novo transcriptome
assembly using three kinds of metrics:
2. read-mapping (if –left and –right are provided)
3. reference-based (if –reference is provided)
Github site to download code is here.
Manual is available here.