<A href=http://genomea.asm.org/content/1/3/e00214-13.full>Noncontiguous Finished Genome Sequence of Staphylococcus aureus KLT6, a Staphylococcal Enterotoxin B-Positive Strain Involved in a Food Poisoning Outbreak in Switzerland</A>
We present the first complete genome sequence of a Staphylococcus aureus strain assigned to clonal complex 12. The strain was isolated in a food poisoning outbreak due to contaminated potato salad in Switzerland in 2009, and it produces staphylococcal enterotoxin B.
<A href=http://www.ncbi.nlm.nih.gov/pubmed/23227987>Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation - Coupland (2012)</A>
We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ng of DNA, offering a significant advantage over current protocols which typically require 400-500 ng of sheared DNA for the library preparation.
Rasko, D.A. et al. Origins of the E. coli strain causing an outbreak of hemolytic-uremic syndrome in Germany. N. Engl. J. Med. 36 5, 709–717 (2011).
Murray, I.A. et al. The methylomes of six bacteria. Nucleic Acids Res. 40, 11450–11462 (2012).
Carneiro, M.O. et al. Pacific Biosciences sequencing technology for genotyping and variation discovery in human data. BMC Genomics 13 , 375 (2012).
<A href=http://genomea.asm.org/content/1/3/e00214-13.full>Noncontiguous Finished Genome Sequence of Staphylococcus aureus KLT6, a Staphylococcal Enterotoxin B-Positive Strain Involved in a Food Poisoning Outbreak in Switzerland</A>