biopython v1.71.0 Bio.Emboss.Primer3
Code to parse output from the EMBOSS eprimer3 program.
As elsewhere in Biopython there are two input functions, read and parse, for single record output and multi-record output. For primer3, a single record object is created for each target sequence and may contain multiple primers.
i.e. If you ran eprimer3 with a single target sequence, use the read function. If you ran eprimer3 with multiple targets, use the parse function to iterate over the retsults.
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parse()
Iterate over primer3 output as Bio.Emboss.Primer3.Record objects.
Link to this function
read()
Parse primer3 output into a Bio.Emboss.Primer3.Record object.
This is for when there is one and only one target sequence. If designing primers for multiple sequences, use the parse function.