biopython v1.71.0 Bio.SeqIO.AbiIO

Bio.SeqIO parser for the ABI format.

ABI is the format used by Applied Biosystem’s sequencing machines to store sequencing results.

For more details on the format specification, visit: http://www.appliedbiosystem.com/support/software_community/ABIF_File_Format.pdf

Link to this section Summary

Functions

Iterator for the Abi file format

Iterator for the Abi file format that yields trimmed SeqRecord objects

Generator that returns directory contents

Trims the sequence using Richard Mott’s modified trimming algorithm

Returns single data value

Link to this section Functions

Iterator for the Abi file format.

Link to this function _AbiTrimIterator()

Iterator for the Abi file format that yields trimmed SeqRecord objects.

Link to this function _abi_parse_header()

Generator that returns directory contents.

Trims the sequence using Richard Mott’s modified trimming algorithm.

Arguments:

- seq_record - SeqRecord object to be trimmed.

Trimmed bases are determined from their segment score, which is a cumulative sum of each base’s score. Base scores are calculated from their quality values.

More about the trimming algorithm: http://www.phrap.org/phredphrap/phred.html http://www.clcbio.com/manual/genomics/Quality_abif_trimming.html

Link to this function _parse_tag_data()

Returns single data value.

Arguments:

  • elem_code - What kind of data
  • elem_num - How many data points
  • raw_data - abi file object from which the tags would be unpacked